Unblur is used to align the frames of movies recorded on an electron microscope to reduce image blurrig due to beam-induced motion. It reads stacks of movies that are stored in MRC/CCP4 format. Unblur generates frame sums that can be used in subsequent image processing steps and optionally applies an exposure-dependent filter to maximize the signal at all resolutions in the frame averages. Movie frame sums can also be calculated using Summovie, which uses the alignment resuls from a prior run of Unblur.
Availability & Restrictions
Versions
The following version of Boost are available on OSC systems:
| Version | Pitzer |
|---|---|
| 1.0.2 |
You can use module spider unblur to view available modules for a given machine. Feel free to contact OSC Help if you need other versions for your work.
Access
Unblur is available to all OSC users. If you have any questions, please contact OSC Help.
Publisher/Vendor/Repository and License Type
This software is subject to Janelia Farm Research Campus Software Copyright 1.1. Full details of this license can be found using this link.
Usage
Usage on Pitzer
Set-up
To configure your environment for use of Unblur, run the following command: module load unblur. The default version will be loaded.
To select a particular Unblur version, use module load unblur/version. For example, use module load unblur/1.0.2 to load Unblur 1.0.2.
Further Reading
